Thomas A. Neubert

Associate Professor, Skirball Institute of Biomolecular Medicine, Structural Biology. Director - Proteomics Core. Department of Biochemistry and Molecular Pharmacology

Ph.D., 1990 Johns Hopkins University

Lab Website: 

Keywords: Mass spectrometry, Posttranslational Modifications, Neuroscience, Cell Signaling, Biomarkers

Contact Information: 

Skirball Institute of Biomolecular Medicine
540 First Avenue 5th floor, Lab 18
New York, N.Y. 10016
Office Tel: (212) 263-7265
Lab Tel: (212) 263-7266
Fax: (212) 263-8214

Administrative Contact:

Anne Ng
Tel: (212)263-8573

Using Mass Spectrometry to Study Proteins and Cell Signaling

The main focus of our lab is the development and use of mass spectrometric methods for the study of proteins and their roles in cellular signaling events. While great strides in understanding intracellular signal transduction have been made in recent years by using molecular biological techniques, we feel that a more complete understanding of the dynamics of intracellular decision-making processes can be gained only by studying the proteins directly. We use mass spectrometry as the main tool for our studies because of the wide variety of information about protein structure it can provide while requiring only small amounts of protein for analysis. We feel that the most efficient use of our technology is achieved by collaborating with outstanding molecular neuroscientists through the Protein Mass Spectrometry Core for Neuroscience.

Much of our work involves using stable isotope labeling by amino acids in cell culture (SILAC) and methods developed in our lab to study nerve growth factor signaling in primary neurons. One approach is to identify proteins become phosphorylated or bind to phosphorylated proteins in response to neurotrophins or other signaling molecules. We have also developed and regularly employ methods to study posttranslational modifications of proteins, and their roles in cell signaling. We have also developed and used methods to identify and verify protein and peptide biomarkers for the early detection of such diseases as cancer and cardiomyopathy.

Selected Publications:

Zhang, Guoan, David Fenyö, and Thomas A. Neubert (2008). Screening for EphB signaling effectors using SILAC with a linear ion trap-Orbitrap mass spectrometer. J Proteome Research, 7, 4715 - 4726. PMID: 18816084

Zhang, Guoan, David Fenyö, and Thomas A. Neubert (2009). Evaluation of the variation in sample preparation for comparative proteomics using stable isotope labeling by amino acids in cell culture. J Proteome Research, 8, 1285–1292. PMID: 19140678

Guoan Zhang, Katrin Deinhardt, Moses V. Chao and Thomas A. Neubert (2011). ­­­­Study of neurotrophin 3 signaling in primary cultured neurons using multiplex stable isotope labeling with amino acids in cell culture. J. Proteome Res., 10, 2546-2554. PMID: 21370927

Guoan Zhang and Thomas A. Neubert. Comparison of three quantitative phosphoproteomic strategies to study receptor tyrosine kinase signaling (2011). J. Proteome Res. 10, 5454-62. PMID: 22013880

Guoan Zhang, Thomas A. Neubert, and Bryen A. Jordan (2012). RNA binding proteins accumulate at the postsynaptic density with synaptic activity. J Neuroscience, 32, 599-609. PMID: 22238095

Click here to see all publications in PubMed