Matthias Stadtfeld

Assistant Professor, Skirball Institute of Biomolecular Medicine, Developmental Genetics. Department of Cell Biology

Ph.D., 2005 Albert Einstein College of Medicine

Keywords:Induced Pluripotency, Nuclear Reprogramming, Genomic Imprinting, Hematopoiesis,

 

 

Contact Information: 

Skirball Institute of Biomolecular Medicine
540 First Avenue 4th floor, Lab 1
New York, N.Y. 10016
Office Tel: (646) 501-6750
Lab Tel: (646) 501-6751
Fax: (212) 263-7760
E-mail: matthias.stadtfeld@med.nyu.edu

Administrative Contact:

Anne Ng
Tel: (212) 263-8573
Email: anne.ng@nyumc.org


Reprogramming and mammalian stem cells

Pluripotent cells that have the unique ability to form all cell types of the adult body can be derived in two different ways: 1) by explanting early mammalian embryos,thereby giving rise to embryonic stem (ES) cells and 2) by the enforced expression of defined embryonic transcription factors in adult somatic cells, giving rise to induced pluripotent stem (iPS) cells. The latter process is commonly referred to as reprogramming and allows for the comparatively straightforward generation of patient-specific pluripotent stem cells to study, and ultimately possibly treat, degenerative disorders. In addition, iPSC technology represents a tractable experimental approach to study mammalian development.

Research in my laboratory uses reprogramming technology to identify mechanisms that control gene expression and determine cellular identity, using the mouse as the main model organism. We are working towards a better understanding of the process of induced pluripotency on a cellular and molecular level, including the coordination of remodeling events during reprogramming and the reasons for the occurrence of epigenetic abnormalities in iPS cells. A second major goal is using pluripotent cells for the in vitro generation of adult-type stem cells that are functionally equivalent to their in vivo counterparts found in the body. We are especially interested in understanding the molecular determinants ofblood cell specification and, ultimately, the generation of functional hematopoietic stem cells from ES cells and iPS cells. Finally, we are interested in the molecular regulation of genomic imprinting and the role of imprinted genes in adult stem cells.

Selected Publications:

Stadtfeld M*, Apostolou E*, Ferrari F, Choi J, Walsh RM, Chen T, Oi S, Kim SY, Bestor T, Shioda T, Park PJ, Hochedlinger K., Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells. Nature Genetics. 2012 Mar; 44(4):398-405. PMID: 22387999

Stadtfeld M and Hochedlinger K. Induced pluripotency: history, mechanisms & applications. Genes Dev. 2010 Oct; 24: 2239-63. Review. PMID: 20952534

Stadtfeld M*, Apostolou E*, Akutsu H, Fukuda A, Follett P, Natesan S, Kono T, Shioda T, Hochedlinger K. Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells. Nature. 2010 May 13;465(7295):175-81. PMID: 20418860

Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K. Induced Pluripotent Stem Cells Generated Without Viral Integration. Science. 2008 Nov 7;322(5903):945-9. PMID: 18818365

Stadtfeld M, Maherali N, Breault DT, Hochedlinger K. Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell, 2008 Mar 6;2 (3):230-40. PMID: 18371448

*denotes equal contribution

 

Click here to see all publications in PubMed