Daniel H. Turnbull

Professor, Skirball Institute of Biomolecular Medicine, Structural Biology. Departments of Radiology (CBI) and Pathology. Dir Biomedical Imaging Grad Program

Ph.D., 1991 University of Toronto

Lab Website: http://saturn.med.nyu.edu/research/sb/turnbulllab

Keywords: MRI; Ultrasound; Mouse Embryo; Neonate; Genetic Reporters

 

Contact Information: 

Skirball Institute of Biomolecular Medicine
540 First Avenue 5th floor, Lab 6-7
New York, N.Y. 10016
Office Tel: (212) 263-7262
Lab Tel: (212) 263-7261
Fax: (212) 263-8214
E-mail: daniel.turnbull@med.nyu.edu

Administrative Contact:

Zachary Klush
Tel: (212) 263-3261
Email: zachary.klush@med.nyu.edu


In Vivo Micro-Imaging of Mouse Brain and Cardiovascular Development

Extensive genetic information and the availability of techniques to manipulate the genome of the mouse have led to its widespread use in studies of development and to model human diseases. In this rapid proliferation of methods to genetically engineer mice, in vivo technologies to analyze structural and functional phenotypes in mutant mouse are critical. Noninvasive imaging methods such as ultrasound and magnetic resonance imaging (MRI) are essential clinical tools for assessing fetal health, and for diagnosing and staging many human diseases including cancer, heart disease and many neuro-degenerative disorders. We are developing a combination of ultrasound and MRI microimaging approaches with sufficient resolution and sensitivity to provide noninvasive structural, functional and molecular data on developmental and disease processes in normal and genetically-engineered mice.

Our laboratory pioneered ultrasound imaging and Doppler blood flow approaches for in vivo analysis of mouse cardiovascular development from the earliest embryonic stages of cardiac function. Recently, we have explored the use of intravascular contrast agents to produce high-resolution, 3D vascular images with both ultrasound and MRI. These approaches are currently being extended to enable cell-specific vascular imaging, using contrast agents targeted to membrane proteins expressed in endothelial cells of transgenic mouse embryos. Taken together, these methods are providing in vivo approaches for analyzing cardiovascular development in the mouse, from structure and function at the whole organism level, to the cellular and molecular levels through targeted contrast-enhanced imaging.

In the mouse nervous system, we have developed a unique set of in vivo analytic tools based on MRI and ultrasound imaging technologies. Ultrasound has been developed as a tool for in utero imaging and image-guided injection, enabling cell transplantation and genetic gain-of-function studies with retroviruses. MRI methods are providing a wealth of 3D anatomical and functional data from the fetal to adult mouse brain, allowing longitudinal analysis of the progression of brain cancer, neuro-degenerative disease models, and in vivo phenotype analysis of a wide range of neurological mouse mutants. Moving beyond anatomical studies, we have developed functional MRI methods, including manganese-enhanced MRI for neural activity mapping in the mouse brain from early postnatal stages. Currently, we are developing several MRI methods for stem cell labeling and tracking, including externally applied nano-to-micrometer sized iron-oxide particles for in vivo cell tagging, and transgenic expression of proteins involved in metal internalization and storage as a genetic reporter system for cellular imaging in the developing mouse brain.

Selected Publications:

Bartelle BB, Berrios-Otero CA, Rodriguez JJ, Friedland AE, Aristizábal O, Turnbull DH (2012). Novel genetic approach for in vivo vascular imaging in mice. Circ Res 110: 938-47. PMID: 22374133

Nieman BJ, Shyu JY, Rodriguez JJ, Garcia AD, Joyner AL, Turnbull DH (2010). In vivo MRI of neural cell migration dynamics in the mouse brain. NeuroImage 50: 456-64. PMID: 20053381

Berrios-Otero CA, Wadghiri YZ, Nieman BJ, Joyner AL, Turnbull DH (2009). Three-dimensional micro-MRI analysis of cerebral artery development in mouse embryos. Magn Reson Med 62: 1431-39. PMID: 19859945

Yu X, Sanes DH, Aristizábal O, Wadghiri YZ, Turnbull DH (2007). Large-scale reorganization of the tonotopic map in mouse auditory midbrain revealed by MRI. Proc Natl Acad Sci USA 104: 12193-98. PMID: 17620614

Yu X, Wadghiri YZ, Sanes DH, Turnbull DH (2005). In vivo auditory brain mapping in mice with Mn-enhanced MRI. Nature Neurosci 8: 961-68. PMID: 15924136

Click here to see all publications in PubMed