Matthias Stadtfeld

Assistant Professor, Skirball Institute of Biomolecular Medicine, Developmental Genetics. Department of Cell Biology

Ph.D., 2005 Albert Einstein College of Medicine

LAB WEBSITE:
Stadtfeld Lab
KEYWORDS:
Induced Pluripotency, Nuclear Reprogramming, Genomic Imprinting, Hematopoiesis, Transcription factors

Contact Information

Skirball Institute of Biomolecular Medicine
540 First Avenue 4th floor, Lab 1
New York, N.Y. 10016
Office Tel: (646) 501-6750
Lab Tel: (646) 501-6751
Fax: (212) 263-7760
E-mail: matthias.stadtfeld@med.nyu.edu

Admin Contact

Alberta Chan
Tel: (212) 263-8573
Email: alberta.chan@nyumc.org


Reprogramming and mammalian stem cells

Pluripotent cells that have the unique ability to form all cell types of the adult body can be derived in two different ways: 1) by explanting early mammalian embryos, thereby giving rise to embryonic stem (ES) cells and 2) by the enforced expression of defined embryonic transcription factors in adult somatic cells, giving rise to induced pluripotent stem (iPS) cells.  The latter process is commonly referred to as reprogramming and allows for the comparatively straightforward generation of patient-specific pluripotent stem cells to study, and ultimately possibly treat, degenerative disorders. In addition, iPSC technology represents a tractable experimental approach to study mammalian development.  

In addition to working towards a better understanding of the cellular and molecular mechanisms that allow the induction of pluripotency in essentially any somatic cell type, my laboratory uses reprogramming technology as a tool to probe the epigenetic regulation of gene loci that are subject to genomic imprinting. For this, we are using the study of genetically engineered mouse models as our primary experimental approach. We are especially interested in the reasons for the observed instability of genomic imprinting in certain pathological and possibly physiological conditions and in the consequences of their dysregulation for the organism. A second major goal of the laboratory is focused on identifying molecular roadblocks for the specification of blood cells in the mammalian embryo as well as in cell culture. Ultimately, we would like to use pluripotent cells for the in vitro generation of blood stem and progenitor cells that are functionally equivalent to their in vivo counterparts.

Selected Publications: 
  • Vidal SE, Amlani B, Chen T, Tsirigos A, Stadtfeld M. Combinatorial modulation of signaling pathways reveals cell-type-specific requirements for highly efficient and synchronous iPSC reprogramming. Stem Cell Reports. 2014 Oct 14;3(4):574-84. PMID: 25358786
  • Trapkov V, Stadtfeld M. Panning data for gold: the search for master regulators of cell fate. Nat Methods. 2013 Jun;10(6):476-7. PMID: 23722206
  • Stadtfeld M*, Apostolou E*, Ferrari F, Choi J, Walsh RM, Chen T, Oi S, Kim SY, Bestor T, Shioda T, Park PJ, Hochedlinger K., Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells. Nature Genetics. 2012 Mar; 44(4):398-405. PMID: 22387999
  • Stadtfeld M and Hochedlinger K. Induced pluripotency: history, mechanisms & applications. Genes Dev. 2010 Oct; 24: 2239-63. Review. PMID: 20952534
  • Stadtfeld M*, Apostolou E*, Akutsu H, Fukuda A, Follett P, Natesan S, Kono T, Shioda T, Hochedlinger K. Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells. Nature. 2010 May 13;465(7295):175-81. PMID: 20418860

*denotes equal contribution  

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