Electron Microscopy Laboratory

!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN" "http://www.w3.org/TR/1999/REC-html401-19991224/loose.dtd"> Electron Microscopy Laboratory

Stokes Lab Home Page



Electron Microscopy



	CM200 SIGN-UP



	CM12 SIGN-UP



	Usage Log



	CM200FEG TEM



	CM12 TEM



	Film Scanner



	Optical Diffractometer



	Cryo Stage



	Cryo Plunger



	Field Compensation



	Vibration Compensation



	Admin Information

CM200 FEG Microscope

This is our Philips CM200 cryoelectron microscope for high resolution structure determination with field emission gun, which is operated at 200 kV. The microscope is equipped with a slow scan 1kx1k CCD camera (Gatan 794) and a TV-rate image intensified camera (Gatan 626). We have several cryoholders (Oxford 3500 and Gatan 626). Digital images can be aquired either with Gatan Digital Micrograph or through TVIPS EMMenu 3.0, the latter of which is used for collecting tomographic tilt series.

This microscope is distinguished by its Field Emission Gun (FEG) and Computer-controled Sample Stage (CompuStage). The FEG drastically increases beam coherence and brightness, substantially improving image quality with much higher resolution. The CompuStage allows the automated image acquisition required for electron tomography and also allows users to memorize specific specimen locations when searching for the best place to record their data.

Please note calibrations etc in the CM200 Info Bank on the right hand border of this page

Main Features of the Microscope

High Tension

20 - 200kV in 40-kV increments

Extractor Voltage

Set at 4 kV maximum

Magnification

50 - 750,000x

Camera Length

30 - 265mm

Resolution

0.27nm(point), 0.14nm(line)

Column Vacuum

Ultimate Pressure = 2.7x10-5Pa (IGP = 18)

CompuStage

X-Y Movement:
Max. travel: 2mm
Max. speed: 0.08mm/s.
Step resolution: 2nm
Reproducibility: 500nm
Drift: 1nm/2min
Loop: 100nm

Z Movement:
0.75mm
10mm/s.
0.036mm
0.5um
Meas. resolution: 7nm

Tilt Movement:
+-45º
10º/s.
0.04º/s.
0.5º
0.01º
Eucentricity: 1.0um

Focussing

0.3um-10mm(LM Range), 3nm-100nm(M/SA Range)

Spot Size

10um-200nm(LM Mode), 2.0um-40nm(Microprobe), 100nm-2nm(Nanoprobe)

CM200 Info Bank
	Basic Procedures
	Magnification
	Defocus Calibration
	CCD Response
	Faraday Cage
	Problems & Solutions
	Spot-scan problem
	Stokes CM200 Notes

Resources

	print this page
	e-mail this page
	seminars


	The Helen L. and Martin S. Kimmel Center for Biology and Medicine at the Skirball Institute for Biomolecular Medicine 540 First Avenue, NYC 10016 ©2004 New York University Contact the Webmaster \| Ethics \| Disclaimer	Home \| Research \| Education \| Facilities \| Administration