Tomgraphy of desmosomes
Tomogram of desmosome induced in cultured keratinocytes.
Skin cells cultured and samples prepared at NYU. Images recorded at
UCSF in Agard's lab with EM430 at 23kx magnification. 3D reconstruction
done with Priism and movie made with IMOD.
Aligned images of tilt series (1.1 Mbyte MPEG
movie)
Sections through the tilt series (0.7 Mbyte MPEG
movie) 
Tomogram of microvilli from supporting cell of frog
inner ear saccula.
Samples prepared by Manfred Auer. Images recorded at NYU on our
CM200FEG microscope at 20kx magnification using EMACT control software.
3D reconstruction determined with EM3D and movie made with IMOD.
Aligned images of tilt series (1.1 Mbyte MPEG
movie) 
Sections through the tilt series (0.4 Mbyte MPEG
movie) 
Partial segmentation of this tomogram using EM3D. Closeup on right
shows substructure along actin filaments, possibly reflecting actin monomers,
and regular connections to the membrane surface, probably corresponding
to myosin.
Tomogram of collagen filaments in extracellular matrix
of frog inner ear saccula.
Samples prepared by Manfred Auer. Images recorded at NYU on our
CM200FEG microscope at 38kx using EMACT control software. 3D reconstruction
determined with EM3D and movie made with IMOD.
Aligned images of tilt series (2.4 Mbyte MPEG
movie) 
Sections through the tilt series (1.0 Mbyte MPEG
movie) 